|
|
Study of 3D bacterial cellulose production using banana peels as a carbon source
Netopilík, Tibor ; Pernicová, Iva (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis deals with the study of 3D bacterial cellulose production using Komagataeibacter xylinus using banana peels as a carbon source. The theoretical part deals with the comparison of the properties of bacterial cellulose and plant cellulose, different methods of biotechnological production of bacterial cellulose and its use. The aim of the bachelor thesis was to find out whether it is possible to use banana peel as a carbon source for biotechnological production of bacterial cellulose. Banana peels are waste lignocellulosic material produced, for example, in the production of snacks or fruit or dairy beverages in the food industry. HPLC analysis showed that 1 l of hydrolyzate after enzymatic hydrolysis of 100 g of dry banana peels per 1 l of water contained 8.86 g of glucose and 10.46 g of fructose. The hydrolyzate was used as a carbon source for static and dynamic cultivation of Komagataeibacter xylinus. The yields of bacterial cellulose produced by using banana peels or glucose were compared. The use of banana peels hydrolyzate increased the yields of bacterial cellulose about 170 % in the case of static cultivation and about 220 % in the case of dynamic cultivation. Scanning electron micrographs of bacterial cellulose confirmed the morphological differences between bacterial cellulose produced statically and dynamically. Moreover, the morphology of bacterial cellulose was influenced by the kind of used carbon source.
|
|
|
Modification of porosity of bacterial cellulose in situ
Ondruchová, Barbora ; Pernicová, Iva (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis deals with the production and modification of porosity of bacterial cellulose in situ using the bacterium Komagataeibacter xylinus. The theoretical part of the work was focused on the review of various methods of culturing Komagataeibacter xylinus and the production of porous samples of bacterial cellulose. The sizes of pores in bacterial cellulose depend mainly on the applied cultivation method. Bacterial cellulose produced statically or dynamically contains pores with the dimensions of approximately 0.02 µm to 10 µm. The difference in porosity in bacterial cellulose prepared by static and dynamic cultivation was confirmed experimentally. The production yields of bacterial cellulose were compared and discussed. Next, the porosity of the bacterial cellulose was modified in situ by the addition of wax particles. Scanning electron microscopy confirmed, that the accumulation of wax particles in the production medium could significantly support the porosity of bacterial cellulose and, at the same time, increase its production.
|
|
|
Study of 3D bacterial cellulose production using banana peels as a carbon source
Netopilík, Tibor ; Pernicová, Iva (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis deals with the study of 3D bacterial cellulose production using Komagataeibacter xylinus using banana peels as a carbon source. The theoretical part deals with the comparison of the properties of bacterial cellulose and plant cellulose, different methods of biotechnological production of bacterial cellulose and its use. The aim of the bachelor thesis was to find out whether it is possible to use banana peel as a carbon source for biotechnological production of bacterial cellulose. Banana peels are waste lignocellulosic material produced, for example, in the production of snacks or fruit or dairy beverages in the food industry. HPLC analysis showed that 1 l of hydrolyzate after enzymatic hydrolysis of 100 g of dry banana peels per 1 l of water contained 8.86 g of glucose and 10.46 g of fructose. The hydrolyzate was used as a carbon source for static and dynamic cultivation of Komagataeibacter xylinus. The yields of bacterial cellulose produced by using banana peels or glucose were compared. The use of banana peels hydrolyzate increased the yields of bacterial cellulose about 170 % in the case of static cultivation and about 220 % in the case of dynamic cultivation. Scanning electron micrographs of bacterial cellulose confirmed the morphological differences between bacterial cellulose produced statically and dynamically. Moreover, the morphology of bacterial cellulose was influenced by the kind of used carbon source.
|
|
|
Modification of porosity of bacterial cellulose in situ
Ondruchová, Barbora ; Pernicová, Iva (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis deals with the production and modification of porosity of bacterial cellulose in situ using the bacterium Komagataeibacter xylinus. The theoretical part of the work was focused on the review of various methods of culturing Komagataeibacter xylinus and the production of porous samples of bacterial cellulose. The sizes of pores in bacterial cellulose depend mainly on the applied cultivation method. Bacterial cellulose produced statically or dynamically contains pores with the dimensions of approximately 0.02 µm to 10 µm. The difference in porosity in bacterial cellulose prepared by static and dynamic cultivation was confirmed experimentally. The production yields of bacterial cellulose were compared and discussed. Next, the porosity of the bacterial cellulose was modified in situ by the addition of wax particles. Scanning electron microscopy confirmed, that the accumulation of wax particles in the production medium could significantly support the porosity of bacterial cellulose and, at the same time, increase its production.
|
guest ::
